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Fluorescence Spectrometers

fp8700

Designed with the latest technology, the JASCO FP-8000 Series fluorescence spectrofluorometers incorporate the highest sensitivity, fastest spectral scanning capability and excellent analysis-oriented functionality offering integrated solutions for advanced materials research and biochemical analysis applications.

Measurement Systems

FPS3

Absolute quantum yield measurement system

Is a system for measuring quantum yield of fluorescent sample. Various samples such as film, liquid, powder can be measured.

fps1

Stop flow system

It is a system that can mix multiple solutions and measure the reaction process. In addition to fluorescence spectrophotometer, it also supports circular dichroism spectrometer, ultraviolet visible spectrophotometer.

fps4

Near infrared fluorescence measurement system

It is a near infrared fluorescence measurement system that can extend the measurement wavelength to 1700 nm or 1400 nm. It is also possible to measure the quantum efficiency in the near infrared region.

Circularly Polarized Luminescence Spectrophotometer

Circularly polarized luminescence (CPL) spectra are said to contain important information for chemical reactions via excited states, such as the stable structure of excited molecules and their behaviors. 

FP-8000 Series Models

Spectrofluorometers and accessories to meet all your needs of measuring for a wide range of applications from biological to advanced materials.

The FP-8200 Spectrofluorometer is the basic, compact model for routine measurements such as spectral scanning and quantitation.

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The FP-8300 Spectrofluorometer is a sophisticated optical system with additional features for biological applications.

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The FP-8500 spectrofluorometer is exceptionally sensitive with a Raman S/N ratio of 8,500:1 (RMS), which allows for fast measurements of samples with low level fluorescence.

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The FP-8600 with extended wavelength operation into the near infrared.

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The FP-8700 enables fluorescence measurement in the near infrared region. By using a near infrared cooling photomultiplier tube for the fluorescence side spectrometer, it is possible to extend the measurement wavelength to 1700 nm or 1400 nm. Ideal for measuring substances that emit fluorescence in the near infrared range, such as rare earth complexes and carbon nanotubes.

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FP-8000 Series Features

Each FP-8000 Series Fluorometer has an optical bench designed specifically for high sensitivity measurements with a range of features that makes it excellent for applications that require precise data. The FP-8000 Series of spectro-fluorometers has been developed for a variety of applications including biomolecular structure, advanced materials research, and quantum yield measurements:

  • Highest sensitivity (> 8,500, RMS)
  • Fastest scan speed (120,000 nm/min)
  • Wide dynamic range (> 6.5 orders of magnitude)
  • Auto-Gain and Auto-SCS (Sensitivity Control System)
  • Automatic higher-order diffraction cut filter
  • Rapid 3D spectra measurements
  • Expanded features for phosphorescence measurements (minimum lifetime measurement of 1 millisecond)

Features of the FP-8000 Series Fluorometer

  • High throughput optical system
  • Wide dynamic range for high sensitivity measurements (at least 6 orders of magnitude) using Auto Gain and Auto Sensitivity Control System for optimal S/N
  • Variable spectral bandwidth down to 1 nm to resolve narrow spectral features
  • Automatic band-pass filters for higher order diffraction
  • High speed scanning for rapid data acquisition
  • Spectral Correction for Quantum Yield measurements

Unique Features and Accessories

  • Range of precise temperature control accessories
  • Automatic polarizers for anisotropy measurements (with temperature control)
  • Integrating spheres for Quantum Yield measurements
  • Microplate Reader for rapid sample throughput
  • Microsampling accessories for small volume samples
  • Stopped-flow system for rapid kinetics
  • Spectra Manager™ Suite software for control and data analysis
  • Flexible design allows for field upgrades for different measurements  as applications and budgets evolve

Versatility for a Wide Range of Applications

Fluorescence applications including: Protein dynamics, Quantitative analysis, Cellular membrane studies, Enzyme kinetics, Carbon nanostructures, Characterization of fluorescent probes, Short life-time phosphorescence measurements, Thermal stability measurements, High Temperature and Up-Conversion.

Materials & Analytics Applications

Evaluation of FRET as a Sensitive Biosensor for Explosives

Fluorescence resonance energy transfer (FRET) is a mechanism governing the energy transfer between two neighboring molecules.

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Absolute Quantum Yields Using an Integrating Sphere

Different molecular and environmental conditions not only effect whether a molecule will fluoresce or not, but can also determine the intensity of the emitted fluorescence radiation.

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Internal Quantum Efficiency Measurement of Phosphor Powders

Some white LEDs consist of two types of materials: a luminescent diode that emits a blue light in the near-UV region, and a phosphor that emits visible light from the absorption to the near-UV region. 

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Remote Fluorescent Measurement using FP-8500 Fluorometer with Optical Fiber

A fluorometer with optical fiber can be used for a variety of measurements, such as when the sample is larger than the sample chamber, to follow an in-situ reaction, or for measurement in hazardous environments, such as high/low temperature and high pressure.

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Quantum Yield Measurement of the Up-Conversion Phosphors

Quantum yield is very useful in determining the efficiency of multi-step up-conversion excitation process that results in fluorescence at shorter wavelengths than the excitation light. Molecules can be excited at longer wavelengths where light scattering and absorption do not occur.

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Life Sciences Applications

Determination of the Relative Quantum Yield of Rhodamine-B

Different molecular and environmental conditions not only effect whether a molecule will fluoresce or not, but can also determine the intensity of the emitted fluorescence radiation.

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Micro-volume Analysis of a λ DNA Using a One Drop Accessory

For many biological samples, it is much more convenient, cost effective, and efficient to use microvolumes for structural and quantitative studies.

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Near-Infrared Phosphor Measurement

Near-infrared fluorescence labels are currently attracting the attention of the scientific community due to their wide range of biological applications.

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Measurement of Trypsin Activity Using a Fluorescence Peptide Substrate

In biology, proteolytic reactions occur to regulate enzyme and protein activity for metabolic to signal processing functions. Proteases are responsible for breaking down biological molecules into smaller polypeptide chains through hydrolysis. 

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Food & Beverages Application

High-Speed Excitation Emission Matrix Measurement of Olive Oil

Excitation-Emission Matrix (EEM) is used with fluorescence spectroscopy for identification and characterization in mixture analysis. Recently, EEM has started to become an accepted method for determining the type, quality, and geographical origin of food products.

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Environmental Application

Component Analysis of an Excitation-Emission Matrix of Environmental Water Samples Using PARAFAC Analysis

Parallel Factor Analysis (PARAFAC) is a type of multivariable analysis, which can be used to extract the components from mixed 3D fluorescence data. JASCO has developed an advanced solution using PARAFAC, which can help to interpret the complicated data found in the EEM.

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Spectrometer Specification

 

Model FP-8200 FP-8300 FP-8500 FP-8600 FP-8700
Light Source Continuous output Xe arc lamp with shielded lamp housing (150 W)
Light Source (for validation) Integrated, selectable low pressure mercury lamp
Photometric System Radio-photometer system using monochromatic light to monitor the intensity output of the Xe lamp
Monochromator Holographic concave grating in modified Rowland mount
Wavelength Range (with Standard Detector)
Ex Zero order, 200 - 750 nm Zero order, 200 - 850 nm Zero order, 
200 - 850 nm
Em Zero order, 200 - 1010 nm Zero order, 
300 - 1400 nm
Wavelength Range (Optional) Zero order, 200 - 900 nm Zero order, 200 - 850 nm N/A option 1700nm
Automatic Cut Filters for High Order Diffraction Option Standard
Sensitivity *1 (RMS)
Peak *2 380 : 1 680 : 1 1,200 : 1 600 : 1  
Base *3 4,500 : 1 2,800 : 1 8,500 : 1 3,500 : 1 1400 nm - 8,000:1
1700 nm - 1,000:1
Resolution
Ex 2.5 nm (at 546.1 nm) 1.0 nm (at 546.1 nm) 1.0 nm (at 546.1 nm) 1.0 nm (at 546.1 nm)
Em 2.5 nm (at 546.1 nm) 1.0 nm (at 546.1 nm) 1.0 nm (at 546.1 nm) 2.0 nm (at 546.1 nm)  
Band Width
Ex 2.5, 5, 10, 20 nm 1, 2.5, 5, 10, 20 nm 1, 2.5, 5, 10, 20, L5, L10 nm 1, 2.5, 5, 10, 20, L5, L10 nm
Em 2.5, 10, 20, 40, L10, L20 nm
Wavelength Accuracy
Ex ±2.0 nm ±1.5 nm ±1.0 nm ±1.0 nm
Em ±2.0 nm
Wavelength Repeatability
Ex ±1.5 nm ±1 nm ±0.3 nm ±0.3 nm
Em ±0.6 nm
Wavelength Scan Speed
Ex 20, 50, 100, 200, 500, 1,000, 2,000, 5,000, 
10,000, 20,000 nm/min
10, 20, 50, 100, 200, 500, 1,000, 2,000, 5,000, 10,000, 20,000, 
60,000 nm/min
10, 20, 50, 100, 200, 500, 1,000, 2,000, 5,000, 10,000, 20,000, 60,000 nm/min
Em 20, 50, 100, 200, 500, 1,000, 2,000, 5,000, 10,000, 20,000, 60,000, 120,000 nm/min
Slew Speed
Ex 30,000 nm/min 60,000 nm/min 60,000 nm/min
Em 30,000 nm/min 120,000 nm/min
Response 20, 50, 100, 200, 
500 msec, 1, 2, 4, 8 sec
10, 20, 50, 100, 200, 500 msec, 1, 2, 4, 8 sec
Detector Ex: Silicon photodiode, Em: PMT Ex: Silicon PD, Em: LN2 Cooled PMT
Photometric Range -10,000 - 10,000
Sensitivity Selection High, Medium, Low, Very Low, Manual, Auto SCS
Auto Gain Standard
Shutter Function Standard (Automatic control)
Sample Illuminating System Horizontal illumination
Sample Compartment 10 mm rectangular cell holder, nitrogen purgeable
Recognition of IQ Accessory Standard
Start Button Standard
Analog Output Standard
Instrument Communication USB 2.0
Control and Data Processing Spectra Manager™/CFR, iRM Spectra Manager™/CFR
Spectral Correction Option Standard
(Spectral correction using a Rhodamine B ethylene glycol solution is standard; other jigs for spectral correction are available separately as options.)
Dimensions 490(W) × 545(D) × 270(H) mm 520(W) × 545(D) × 270(H) mm 570(W) × 545(D) × 270(H) mm  
Weight 33.6 kg (74 lbs) 36 kg (79 lbs) 39 kg (86 lbs) 40 kg (88 lbs)
Power Requirement 270VA
Installation Environment Temperature: 15 to 35°C, Humidity: Less than 85%

*1 : Minimum Signal-to-Noise ratio of Raman band of water, excitation 350 nm, band width Ex 5 nm Em 5 nm (FP-8600: Ex 5 nm Em 10 nm), response 2 seconds.
*2 : Noise is measured on the Raman peak.
*3 : Noise is measured on the baseline.

Software Specification

A wide range of software applications and accessories can be used with Spectra Manager™ Suite.

Model FP-8200 FP-8300 FP-8500 FP-8600
Data Station Type Spectra Manager™/Spectra Manager™ CFR (Microsoft Windows® 7 or 8.1 Professional)
iRM Type Handheld Intelligent Remote Module iRM-900 N/A
Measurement Programs
Spectra Manager™

Spectra measurement, 

Quantitative measurement, 

Fixed wavelength measurement, 

Time course measurement, 

3-D Spectra measurement, 

Abs measurement

Spectra measurement, 

Quantitative measurement, 

Fixed wavelength measurement, 

Time course measurement, 

3-D Spectra measurement, 

Abs measurement, 

Phosphorescence measurement

Spectra Manager™ CFR *4
iRM-900 *5 Spectra measurement, Quantitative measurement, Fixed wavelength measurement, Time course measurement, 3D Spectra measurement, Abs measurement N/A
Spectra Correction Program Standard *6 Standard
Instrument Validation Program and Hg lamp (Standard), Accessories (option) Program and Hg lamp (Standard), Accessories (Standard)
Self Diagnosis Standard
IQ Accessory Recognition and IQ Start Standard

*4 : Spectra Manager™ CFR is the 21 CFR Part 11 compliant software package.
*5 : The iRM-900 handheld control module can be used with any PictBridge compatible printer.
*6 : Optional components are necessary.
Specifications are subject to change without notice.

High speed 3D spectral measurement with the highest class wavelength scanning speed

3D spectra measurement is available for all models of the FP-8000 series. The analysis software offers a variety of processing methods to easily display the relevant data characteristics.

3Dscan1

3D fluorescence spectrum of quinine sulfate

3Dscan2

Fluorescence 3D spectrum of erbium oxide

3Dscan3

 NIR three-dimensional fluorescence spectrum of single-walled carbon nanotube

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Details

Highest S/N performance

The high S/N (signal-to-noise) performance of the FP-8000 series is achieved by a high throughput optical system and low-noise signal processing.

8500:1 or greater (RMS, FP-8500)

Spectra of fluorescein solutions

Spectra of fluorescein solutions

Wide Dynamic Range using Auto-Gain and Auto-SCS

A wide dynamic range for luminescence measurements is obtained using the Auto-Gain and Auto-SCS features, automatically adjusting the detector sensitivity for optimum measurements.

Auto-SCS

Effective for fixed wavelength measurements and quantitative analysis, Auto-SCS makes it possible to create the calibration curve for a wide range of concentrations without modifying the instrument measurement parameters.

Calibration curve of fluorescein solutions

Calibration curve of fluorescein solutions

Auto-Gain

Collecting data with an optimized S/N throughout the entire scan range for spectra or time course measurements is obtained with ease using the Auto-Gain feature, automatically adjusting the gain due to fluorescence intensity.

Spectra of quinine sulfate solution

Spectra of quinine sulfate solution

Automatic higher-order diffraction cut filter

The conventional method for removing higher-order diffraction artifacts from excitation/emission spectra involves selection and installation of the proper cut filters according to the scanning wavelengths. The automatic cut filter system of the FP-8000 series (option for FP-8200) selects the proper cut-off filters for spectral measurements to obtain spectra without higher-order diffraction interference.

Spectra of fluorescent orange color plate

Spectra of fluorescent orange color plate

3D Spectra Measurement

3D spectra measurement is available for all models of the FP-8000 series. The fastest scan speed of 120,000 nm/min for the FP-8500 offers 3D spectral measurement in the shortest time available for any instrument in this class. The analysis software offers a variety of processing methods to easily display the relevant data characteristics.

3D spectra measurement of fluorescent orange color plate

3D spectra measurement of fluorescent orange color plate

Phosphorescence measurement

A high-speed chopper for the FP-8300, 8500 and 8600 instruments offers phosphorescence spectrum measurements as well as advanced phosphorescent lifetime and quantitative analysis measurements.

Luminescence spectra of cooled Benzene

Luminescence spectra of cooled Benzene

The luminescence spectra of benzene measured with 255 nm excitation at 77 K. The blue trace in the figure is the normal emission spectrum; the red trace is the phosphorescence spectrum measured using a 5 msec delay.

IQ Accessories (automatic accessory identification)

The FP-8000 IQ accessories utilize a non-contact RFID sensor for automatic recognition by the control software. Accessory information, including accessory name and serial number, is retrieved and saved in the spectral data file. The IQ Start function can be programmed to automatically select a specified control program for simplified sample measurements.

IQ Accessories

IQ accessory identification using a non-contact sensor (RFID)

An abundance of Special Accessories and Programs

A wide variety of accessories and control/analysis programs are designed to integrate analysis methods for various samples and application requirements ranging from biochemical/bioscience to materials research and beyond.

Special Accessories and Programs

A variety of optimized accessories

Accessories

Model: FMH-801 Name: 3 mm micro cell jacket Available for: FP-8200 / 8300 / 8500 / 8600

 
Model: FMH-802 Name: 5 mm micro cell jacket Available for: FP-8200 / 8300 / 8500 / 8600

 
 
Model: FMH-803 Name: Abs. measurement cell block Available for: FP-8200 / 8300 / 8500 / 8600

 
Model: FMH-804 Name: High sensitivity cell block Available for: FP-8200 / 8300 / 8500 / 8600

 
 
Model: FMH-805 Name: Cell block for triangular cell Available for: FP-8200 / 8300 / 8500 / 8600

 
Model: FMH-806 Name: Cell block for rectangular cell Available for: FP-8200 / 8300 / 8500 / 8600

 
 
Model: FMH-808 Name: Solid sample holding block Available for: FP-8200 / 8300 / 8500 / 8600

 
Model: FMH-809 Name: Film holding block Available for: FP-8200 / 8300 / 8500 / 8600

 
 
Model: FMH-810 Name: Powder sample cell block Available for: FP-8200 / 8300 / 8500 / 8600

 
Model: SAF-850 Name: One-drop measurement unit Available for: FP-8200

 
 
Model: SAF-851 Name: One-drop measurement unit Available for: FP-8300 / 8500 / 8600

 
Model: CTS-855 Name: Coumarin Measurement Unit Available for: FP-8300 / 8500 / 8600

 
 
Model: OBF-832 Name: Optical fiber unit Available for: FP-8300 / 8500 / 8600

 
Model: EFA-833 Name: Epi-fluorescence unit Available for: FP-8300 / 8500 / 8600

 
 
Model: CTH-807 Name: Water thermostatted cell block Available for: FP-8200 / 8300 / 8500 / 8600

 
Model: STR-811 Name: Water thermostatted cell holder with stirrer Available for: FP-8200

 
 
Model: STR-812 Name: Water thermostatted cell holder with stirrer Available for: FP-8300 / 8500 / 8600

 
Model: FCT-816 Name: Water thermostatted 4-position cell changer Available for: FP-8200

 
 
Model: FCT-817 Name: Water thermostatted 8-position cell changer Available for: FP-8300 / 8500 / 8600

 
Model: EHC-813 Name: Peltier thermostatted single cell holder (air-cooled) Available for: FP-8300 / 8500 / 8600

 
 
Model: ETC-814 Name: Peltier thermostatted single cell holder (water-cooled) Available for: FP-8200

 
Model: ETC-815 Name: Peltier thermostatted single cell holder (water-cooled) Available for: FP-8300 / 8500 / 8600

 
 
Model: PCT-818 Name: Automatic 4-Position Peltier cell changer (water-cooled) Available for: FP-8300 / 8500 / 8600

 
Model: PMU-830 Name: Liq. N2 cooling unit Available for: FP-8300 / 8500 / 8600

 
 
Model: CSH-831 Name: Cryostat holder Available for: FP-8300 / 8500 / 8600

 
Model: HPC-836 Name: High temperature powder cell unit Available for: FP-8300 / 8500 / 8600

 
 
Model: SHP-819 Name: Peristaltic sipper Available for: FP-8200

 
Model: SHP-820 Name: Peristaltic sipper Available for: FP-8300 / 8500 / 8600

 
 
Model: QFS-821 Name: Vacuum sipper Available for: FP-8200

 
Model: FSC-823 Name: Micro flow cell holder Available for: FP-8200

 
 
Model: FMP-825 Name: Automatic microplate reader Available for: FP-8300 / 8500 / 8600

 
Model: ASU-800 Name: Auto sampler unit Available for: FP-8200 / 8300 / 8500 / 8600

 
 
Model: ASP-849 Name: Syringe pump unit Available for: FP-8200 / 8300 / 8500 / 8600

 
Model: QFS-822 Name: Vacuum sipper Available for: FP-8300 / 8500 / 8600

 
 
Model: FSC-824 Name: Micro flow cell holder Available for: FP-8300 / 8500 / 8600

 
Model: ISF-834 Name: 60mmΦ Integrating sphere unit Available for: FP-8300 / 8500 / 8600

 
 
Model: ILF-835 Name: 100mmΦ Integrating sphere unit Available for: FP-8300 / 8500 / 8600

 
Model: ILFC-847 Name: ILFC-847 Cooled 100mmΦ Integrating sphere unit Available for: FP-8300 / 8500 / 8600

 
 
Model: ESC-842 Name: Calibrated light source – WI Available for: FP-8200 / 8300 / 8500 / 8600

 
Model: ESC-843 Name: Calibrated light source – D 2 Available for: FP-8200 / 8300 / 8500 / 8600

 
 
Model: SID-844 Name: Calibrated detector Available for: FP-8200 / 8300 / 8500 / 8600

 
Model: SFS-852/853/854 Name: Stopped flow system Available for: FP-8300 / 8500 / 8600

 
 
Model: ATS-826 Name: Automatic titration unit Available for: FP-8200 / 8300 / 8500 / 8600

 
Model: CSP-828 Name: Lid with syringe port Available for: FP-8200

 
 
Model: ATS-827 Name: Automatic titration unit Available for: FP-8300 / 8500 / 8600

 
Model: CSP-829 Name: Lid with syringe port Available for: FP-8300 / 8500 /8600

 
 

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